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NC_000913.3_LC-E18
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screen performed in strain LC-E18, E. coli strain MG1655 expressing dCas9 under the control of a Tet promoter integrated at the 186 attB site. Here a strong RBS controls the expression of dCas9 leading to the bad-seed toxicity phenomenon and increased off-target effects.
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LB
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A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
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NC_000913.3_Wang
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Genome-wide screen with a library of ~60,000 guides (~15 guides per gene)
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LB
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Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance
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NC_000913.3_LC-E75
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screen performed in strain LC-E75, E. coli strain MG1655 expressing dCas9 under the control of a Tet promoter integrated at the 186 attB site. Here translation of dCas9 is controlled by a RBS optimized to alleviate the bad-seed toxicity phenomenon.
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LB
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A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
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NC_000913.3_EcoWG1_LB_T4
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screen performed with the EcoWG1 library in strain LC-E75
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LB
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On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria
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