Escherichia coli str. K-12 substr. MG1655, complete genome NC_000913.3
Genome
Screens
Name Description Growth Condition Reference Actions
NC_000913.3_LC-E75 screen performed in strain LC-E75, E. coli strain MG1655 expressing dCas9 under the control of a Tet promoter integrated at the 186 attB site. Here translation of dCas9 is controlled by a RBS optimized to alleviate the bad-seed toxicity phenomenon. LB
A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
Cui, Nat Commun, 2018
NC_000913.3_LC-E18 screen performed in strain LC-E18, E. coli strain MG1655 expressing dCas9 under the control of a Tet promoter integrated at the 186 attB site. Here a strong RBS controls the expression of dCas9 leading to the bad-seed toxicity phenomenon and increased off-target effects. LB
A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
Cui, Nat Commun, 2018
NC_000913.3_Wang Genome-wide screen with a library of ~60,000 guides (~15 guides per gene) LB
Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance
Wang, Nat Commun, 2018
NC_000913.3_EcoWG1_LB_T4 screen performed with the EcoWG1 library in strain LC-E75 LB
On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria
Calvo-Villamañán, Nucleic Acids Res, 2020