Input the sequence of a target gene and this script will output
candidate guide RNAs for the S. pyogenes dCas9 with predicted
on-target activity and an analysis of off-targets. Predictions are
made based on a model of dCas9's ability to block the RNA polymerase
of E. coli
(Calvo-Villamañán et al., 2020)
.
Example
(Escherichia coli str. K-12 substr. MG1655)