Crispr Browser

This is a genome browser to visualize the results of CRISPR-dCas9 screens in bacteria. You can upload your own data or navigate through published datasets.

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Guide RNA design for CRISPRi in bacteria

Input the sequence of a target gene and this script will output candidate guide RNAs for the S. pyogenes dCas9 with predicted on-target activity and an analysis of off-targets. Predictions are made based on a model of dCas9's ability to block the RNA polymerase of E. coli (Calvo-Villamañán et al., 2020) .

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